The Seahorse XF analyzers can detect T cell activation response within minutes of stimulation with CD3/CD28 immunogenic antibodies by measuring the changes in the Proton Efflux Rate (PER, an indicator of glycolysis) in real-time. This unique approach offers important insights into the early dynamics of T cell activation as well as metabolic drivers that shape the outcomes of immune responses.

Download the T cell activation Application Note to learn:

  • The assay design and validation results
  • Application for assessing the activation potential of T cells
  • Demonstration of utility in assessing the acute effect of immune cell modulators

Application Note

"Real-Time Detection and Modulation of Human T Cell Activation Using Agilent Seahorse XF Hu T Cell Activation Assay Kit"

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For Research Use Only. Not for use in diagnostic procedures.

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