The Seahorse XF analyzers can detect T cell activation response within minutes of stimulation with CD3/CD28 immunogenic antibodies by measuring the changes in the Proton Efflux Rate (PER, an indicator of glycolysis) in real-time. This unique approach offers important insights into the early dynamics of T cell activation as well as metabolic drivers that shape the outcomes of immune responses.
Download the T cell activation Application Note to learn:
- The assay design and validation results
- Application for assessing the activation potential of T cells
- Demonstration of utility in assessing the acute effect of immune cell modulators