Adenosine triphosphate (ATP) is the most common high energy intermediate of living organisms. Cells utilize the energy derived from the breakdown of cellular fuels to synthesize ATP from ADP and inorganic phosphate. The free energy released from ATP hydrolysis is then used to sustain various cellular functions such as growth, movement, and response to the environment. Mammalian cells tightly regulate production and consumption of ATP – i.e. supply and demand are balanced to maintain intracellular ATP levels quite constant. Therefore, measuring the rate of ATP production enables a view into cellular function that is not provided by simply measuring the amount of ATP in the cell. The Agilent Seahorse XF Real-Time ATP Production Rate Assay quantifies ATP Production Rates from mitochondrial respiration and glycolysis in real time with live cells providing a unique insight into cellular phenotype and function.
In this webinar we will discuss:
- How the new Seahorse XF Real-Time ATP Production Rate Assay quantifies ATP production from Oxygen Consumption Rate (OCR) and Extracellular Acidification Rates (ECAR) measurements.
- Why ATP Production Rate is more informative than end-point total ATP level assays to provide information about cellular activities and energy demand.
- How to apply the assay to quantify cellular metabolic phenotypes, detect and quantify metabolic switching and uncover pathway or fuel liabilities.
For Research Use Only. Not for use in diagnostic procedures.