Agilent Cell Analysis Neutrophil Activation Application Notes

Quantifying Cellular ATP Production Rate Using Agilent Seahorse XF Technology

Uncover the difference you can bring to your research by exploring examples denoting the contrast in measuring ATP Production Rate that can be achieved using Agilent’s Seahorse XF Real-Time ATP rate assay versus measuring total ATP amounts. 

This white paper describes a  simple approach to quantifying ATP production rate by measuring the two major production pathways in mammalian cells: glycolysis and oxidative phosphorylation. It also covers and validates the necessary calculations to convert the measurement of Oxygen Consumption Rate (OCR; a measure of oxidative phosphorylation) and Extracellular Acidification Rate (ECAR; a measure of glycolysis) to units of ATP Production Rate.


For research use only. Not for use in diagnostic procedures.