Uncover the difference you can bring to your research by exploring examples denoting the contrast in measuring ATP Production Rate that can be achieved using Agilent’s Seahorse XF Real-Time ATP rate assay versus measuring total ATP amounts.
This white paper describes a simple approach to quantifying ATP production rate by measuring the two major production pathways in mammalian cells: glycolysis and oxidative phosphorylation. It also covers and validates the necessary calculations to convert the measurement of Oxygen Consumption Rate (OCR; a measure of oxidative phosphorylation) and Extracellular Acidification Rate (ECAR; a measure of glycolysis) to units of ATP Production Rate.
For research use only. Not for use in diagnostic procedures.